【摘要】 用离体脑片培养和同位素示踪法 ,研究了不同浓度 ( 0 .0 1～ 10 0μmol/L )的孕烯醇酮 ( Pe)和孕烯醇酮硫酸盐( Pes)对小鼠全脑切片摄取 3 H-γ-氨基丁酸 ( 3 H-GABA)的影响 .结果表明 :0 .0 1～ 0 .0 5 μmol/L Pe使 3 H-GABA的摄取量增加 ,而 10～ 10 0μmol/L Pe使摄取量降低 ( P均 <0 .0 0 1) .0 .0 1～ 10μm ol/L Pes对 3 H -GABA的摄取无显著影响 ,而 10 0 μmol/L 的 Pes使摄取量显著增加 ( P<0 .0 0 1) .用印防己毒素、荷包牡丹碱、戊巴比妥钠和巴氯芬分别与 0 .0 1μm ol/L Pe共同使用 ,均能明显增加脑片对 3 H-GABA的摄取 ( P<0 .0 5或 P<0 .0 0 1) .以上结果提示 ,Pe、Pes均能通过影响 GABA的摄取而调节 GABA能系统的活动 ,其影响效应与 Pe、Pes的浓度密切相关 .更多还原

【Abstract】 By using brain slice cultvating and rad iois otope tracing,the effects of different concentration (0.01～100 μmol/L) pregna nolone(Pe)and pregnanolone sulfate(Pes)on the uptake of -3H-γ-Aminobu tyri c Acid ( -3H-GABA) was studied in slices of mice whole brain.The result s howed that 0.01～0.05 μmol/L Pe increased the uptake of -3H-GABA and 10～100 μmo l/L Pe decreased the uptake of -3H-GABA.100 μmol/L Pes markedly increas ed t he uptake of -3H-GABA.The levels of the uptake of 0.01～10 μmol/L P es are nomal.When picrotoxin,bicuculline,pentabarbiturate and baclofen were se parately added to Pe(0.01 μmol/L),the level of uptake of -3H-GABA in th e brain slices was in creased.These results suggested that Pe and Pes could modulate the action of GAB A-ergic system through the GABA uptake system in the mice brain,and the effect was related to the concentration of Pe and Pes.更多还原