【摘要】 检测咬合力在正常及丧失状态下 ,大鼠牙周细胞 TNF— α的动态表达 ,初探 TNF— α在牙周组织改建中的分子机理。方法　采用 HE染色和免疫组化的方法 ,观察牙周形态变化以及牙周组织中 TNF— α蛋白表达。结果　咬合力丧失引起牙周膜结构紊乱、牙槽骨吸收。同时 ,牙周细胞中 TNF— α表达较正常咬合力时明显增强。结论　咬合力丧失 ,促使 TNF—α明显增多。本实验从分子水平探讨了牙周组织改建的机理 ;揭示了牙周组织结构与功能在改建中的一致性。更多还原

【Abstract】 Objective To study the expression of TNF-α in the rat periodontal cells under normal and lost bite force, in order to explain the molecular mechanism of periodontium remodeling. Methods HE staining and immunohistochemistry techniques were applied to study the change in the histologic morphology and the expression of TNF-α protein. Results Under the lost bite force, periodontal ligament structure was disorganized and alveolar bone was absorbed; the expression level of TNF-α increased apparently in periodontal cells.Conclusion Lost bite force induce the expression of TNF-α increased apparently in periodontium. It approached initially the molecular mechanism of periodontium remodeling, suggested that the histologic morphology is closely related to the mechanical condition.更多还原