【摘要】 目的 :观察介入化疗对鼻咽癌细胞凋亡 ( APO)和细胞增殖的影响 ,并评价其疗效。方法 :对 10例鼻咽癌患者经颞浅动脉逆行颈外动脉插管 ,选择肿瘤供血血管灌注化疗药物 5 -氟脲嘧啶、顺铂、平阳霉素及烟酰胺。分别于化疗前、化疗后 7d取肿瘤组织 ,检测凋亡细胞和增殖细胞抗原 ( PCNA)。结果 :介入化疗后临床缓解率为63 %。化疗后 7d细胞凋亡指数 ( AI) 1.2 4% ,高于治疗前的 0 .5 2 %。细胞增殖指数化疗前为 42 .4% ,化疗后 7d为5 1.2 %。呈增高趋势。结论 :鼻咽癌介入化疗可改善局部症状 ,这是通过诱导细胞凋亡而完成的更多还原

【Abstract】 Objective:To investigate the timing changes of apoptosis (APO) and PCNA after intra-arterial infusion chemotherapy for nasopharyngeal carcinoma.Method:Ten patients were subjected to percutaneous superficial temporal artery catheterization and infusion of anticancer drugs:5 Fu,DDp,BLM, nicotinamide.The biopsy of nasopharyngeal tumor tissues was performed before chemotherapy and 7 days after chemotherapy. Apoptotic cells were examined by TOT mediated DUTP fluorescein and labeling. The expression of proliferating cells nuclear antigen (PCNA) was detected with immunohistochemical staining. Result:AI of nasopharyngeal cancer cells before chemotherapy and 7 days after chemotherapy was 0.52% and 1.42%,PI was 42.4% and 51.2%.Conclusion:Intra arterial infusion chemotherapy not only induced apoptosis effectively, but also inhibited temporarily tumor cells proliferation in patients. The curative surgical treatment should be performed as soon as possible after chemotherapy.更多还原