【摘要】 为进一步证实毁损弓状核可诱发动脉粥样硬化的早期变化这一作用 ,用毁损弓状核诱发动脉粥样硬化早期变化后 ,重新植入胚鼠弓状核细胞悬液来观察动脉壁的变化是否能得以恢复。实验用新生Wistar大鼠 18只 ,按出生后 1、3、5、7、9天皮下注射 10 %谷氨酸单钠 4g kg体重 ,共注射 5次 ,为毁损弓状核诱发动脉粥样硬化动物模型 ,存活 30天后 ,取 6只动物主动脉作为对照组Ⅰ ,另 12只动物分为实验组和对照组Ⅱ ,实验组在弓状核区重新植入 14天胚鼠弓状核细胞悬液 5 μL(细胞浓度为 1× 10 1 5 L) ,对照组Ⅱ植入等量生理盐水继续存活 30天后各取主动脉作电镜观察。结果发现 :对照组Ⅰ动物主动脉壁内皮细胞腔面可见微绒毛样突起 ,内皮细胞核有些扭曲 ,不规则 ,内皮下层未见增厚 ;对照组Ⅱ动物内皮细胞变性、脱落 ,内皮下层明显增厚 ,出现许多大小不等的空泡和胶原纤维 ,有巨噬细胞和平滑肌细胞迁入内皮下层 ,这些变化是动脉粥样硬化发病的早期特征性病变 ;实验组动物内皮细胞核完整 ,内皮与内皮下层连接紧密 ,偶尔可见内皮细胞核扭曲。实验证明 ,在被毁损弓状核区重新植入胚鼠弓状核细胞后对动脉粥样硬化有明显恢复作用 ,从而证实下丘脑弓状核对动脉粥样硬化的形成具有重要调控作用更多还原

【Abstract】 Aim It has been proved in our previous work that destruction of arcuate nucleus could induce early atherosclerosis .In order to further research into the influence of hypothalamic arcuate nucleus on the development of atheroscerosis, this study was to investigated whether the transplantation of cell suspension of arcuate nucleus into the destroyed nucleus would act on the recovery of atherosclerosis. Methods Eighteen new born Wistar rats injected with monosodium glutamate(MSG)4 g/kg hypodermically once every other day for 5 d were used as induced atherosclerosis model. After surviving for 30 d, six animals sacrificed with their aorta taken serve as control group Ⅰ. The other 12 animals were equally divided into experiment group and control group Ⅱ. The experimental group were transplanted with 5 μL cell suspension (1×10 15 /L)of arcuate nucleus from 14 d embryo and control groupⅡ were given equal volume of normal saline. After surviving for another 30 d, the aorta of these animals were made ultrathin section and investigated under electromicroscope. Results In control group Ⅰ(arcuate nucleus damaged by MSG for 30 d), microvillous projections of endothelial cells were observed on the luminal surface and the nuclei of the endothelial cells became twisted and irregular, but without thickening of subendothelial tissue. In control group Ⅱ(arcuate nucleus damaged by MSG for 60 d), degeneration of endothelial cells could be seen and some endothelial cells had fallen off. The subendothelial tissue was evidently thickened with a few collagnous fibers, vesicles of various size and macrophage or smooth muscle cells migrated from blood and tunica media through the ruptured elastic intima, indicating characteristic changes of early atherosclerosis. In the experimental group (transplanted with embryonic arcuate nucleus), the endothelial cells remained intact, and connected tightly with subendothelial tissue, occasionally twisted nucleus could be seen. Conclusions This experiment indicate that transplanted arcuate nucleus in the destroyed nucleus has notably made the animal recovery from atherosclerosis and thereby confirms that hypothalamic arcuate nucleus has influence on development and/or control of atherosclerosis.更多还原