【摘要】 目的 研究大鼠同种异体心脏移植模型中细胞间粘附分子－１（ＩＣＡ－１）的表达以及环抱嘌呤Ａ（ＣｓＡ）抗免疫排斥的作用，井探讨该作用的影响因素。方法 根据国际心肺移植组织（ＩＳＨＬＴ）１９９０年心脏急性排异反应分级标准．对心脏移植大鼠进行排异反应分析；用免疫组织化学方法检测ＩＣＡＭ－ｌ的表达部位和表达水平。结果 心脏移植后，供者心脏和主动脉标本中，１ｄ和 ３ｄ时出现轻度炎性浸润，排异反应为 １Ａ或 １Ｂ级；１１ｄ和 １２ｄ时可出现弥谩性炎性浸润和心肌坏死，并伴明显水肿、出血以及血管炎．排斥级别达３Ｂ或４级。ＣＳＡ治疗可使心脏移植排异降低１～２．５级。移植后血管内皮细胞、外膜浸润淋巴细胞的ＩＣＡＭ－１表达显著升高。ＣｓＡ治疗可明显抑制供者心脏及主动脉的ＩＣＡＭ－１表达。该抑制作用在ｌ～３ｄ内，呈ＣｓＡ剂量依赖性．而在７～１１ｄ内无剂量差异。结论ＣｓＡ治疗是下凋淋巴细胞迁移和浸润、减弱移植排斥反应的有效手段．其机理可能与降低粘附分子表达有关。更多还原

【Abstract】 Objective To study the expression of intercellular adhesion molecule-1 (ICAM-1 ) in the rat cardiac heterotopic allo- grafts and the effect of cyclosporin A on prevention of allograft rejection. Methods Heart transplantated animals were divided into three groups: Group Ⅰ(control), Group Ⅱ(CsA 7. 5mg/kg B W, daily) and Group Ⅲ(CsA l5mg/kg B W, daily). Acute cardiac rejection grade was valued by the standrd of ISHLT(1990 ). Immunohistochemistry was performed to analyze the expression of ICAM-1 in heart grafts and donor aorta segments. Results After heart transplantation, it was found that from day 1 to 3, there was sligtly inflammatory infiltratiation, the rejection was graded 1A of 1B, But from day 11 to 12, there were disseminated inflammation and cardiac necrosis with serous hyperemia, exutation, edema, acute vasculitis and myocarditis. The rejection grade was 3B or 4, and could be reduced 1to 2. 5 grades by administration of CsA. It was also found that both in heart graft and donor aorta segments the expression of ICAM-1 on the endothelium cells, infiltrated lymphocytes was clearly increased. It was time-dependent and could be down-regulated by administra- tion of CsA. On Day 1 and Day 3 the suppressing function of CsA on expressing of ICAM-1 showed singificant dosage-dependent. But from Day 7 to Day 11, it appeared dosage- independent. Conclusiou Trea tment with CsA is an effect ive methed to down- regulate I - CAM-1 expression and could reduce the lympphocyte migration and filtration. These results may explain, in part, the mechanism of CsA reducing acute rejection in a rat cardiac transplantation medel.更多还原