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In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

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【Author】 CHEN Chunying, ZHANG PeiqunCHAI Zhifang, LI Guangcheng& HUANG YuyingLaboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China;Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China

【机构】 Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China； ,Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China； ,Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China,Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China；

【摘要】 <正> A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed a-long polyacrylamide gel. The results showed that the protein fraction of peak III in cvtosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.更多还原

【Abstract】 A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed a-long polyacrylamide gel. The results showed that the protein fraction of peak III in cvtosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.更多还原

【关键词】 synchrotron radiation； X-ray fluorescence analysis； in situ analysis； metalloprotein； electrophoretic separation.；
【Key words】 synchrotron radiation； X-ray fluorescence analysis； in situ analysis； metalloprotein； electrophoretic separation.；

【文献出处】 Science in China,Ser.A ,中国科学A辑(英文版) , 编辑部邮箱 ,2000年01期
【分类号】O434.1
【被引频次】1
【下载频次】25

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