【摘要】 从广东番禺某猪场病死仔猪中分离到一株疑为猪伪狂犬病病毒 (PRV)毒株 ,通过对其理化特性的鉴定、中和试验、实验动物回归试验、电镜观察、PCR扩增及感染力测定 ,发现其对氯仿、乙醚均敏感 ;能中和Pr闽A株标准阳性血清 ;实验兔接种该病毒后 ,发生瘙痒并麻痹致死 ;电镜观察可见病毒粒子呈不规则圆形并带有囊膜和纤突 ;PCR体外扩增可见其与闽A株在同一水平位置上有相同的电泳带 ;用Marc14 5细胞测定其毒价为 1 0 7.5TCID50 /mL 根据此分离病毒株的上述特性 ,鉴定其为猪伪狂犬病病毒更多还原

【Abstract】 An isolate suspected to be pseudorabies virus was recovered from dead piglets on a pig farm in Panyu Guangdong. It was characterized by its physicochemical properties, virus neutralization test, animal inoculation, electron microscopy, PCR amplification and in vitro infectivity test. The isolate was found to be sensitive both to chloroform and ether, could be neutralized by the standard pseudorabies Fujian A strain virus antibodies, rabbits inoculated with the isolate developed localized itch and then paralysis before death, electron microscope revealed irregularly round virus particles with envelope and peplomers, PCR amplification of the virus genome yielded a fragment which co migrated with similarly amplified fragment of the Fujian A standard PRV. When titrated in Marc 145 cell culture, the virus titre attained 10 7.5 TCID 50 /mL. The isolate was identified as a pseudorabies virus on the strength of the above findings.更多还原