【摘要】 目的 :将人内皮抑素 (endostatin)基因插入表达载体并进行原核表达。方法 :将 endostatin基因定向插入表达载体 p QE-31Bam H I/ Kpn I位点 ,构建重组质粒 p QEN,转化 E.coli M15 ,进行原核表达。结果 :在 IPTG的诱导下 ,endostatin基因在重组转化株 E.coli M15 (p QEN)中获得高效表达 ,SDS- PAGE显示表达产物分子量为 2 2× 10 3u,表达产物主要以包涵体形式存在 ,表达量占菌体总蛋白的 34 .6 %。结论 :从组织中克隆 endostatin基因并进行原核表达是可行的方法更多还原

【Abstract】 Objective:To obtain recombinant human endostatin by a prokaryotic expression system Methods:Human endostatin gene was cloned on BamH I/Kpn I site of the expression vector pQE 31 to result in the recombinant plasmid pQEN The pQEN was transformed into E coli M15 and was expressed by this prokaryoticexpression system Results:Endostatin was efficiently expressed in recombinant strain E coli M15(pQEN) as inclusion body by IPTG induction The expression product constitutes about 34 6%of the total bacterial protein and was characterized by SDS PAGE to have molecular weights of 22×10 3 u Conclusion:The method of cloning endostatin gene from human fatal liver and express it in a prokaryotic expression system are feasible and useful.更多还原