【摘要】 用荧光光谱和紫外吸收光谱研究了pH =2 3～ 1 1 3范围内牛血清白蛋白 (BSA)芳香氨基酸残基微环境的变化 ,以此推断蛋白质构象的变化 ,并讨论蛋白质表面疏水性变化的趋势。与中性环境相比 ,pH为 2 3时 ,色氨酸微环境的疏水性增强 ,蛋白质局部表面疏水降低 ;pH为 1 1 3时 ,部分Phe残基微环境的极性增强 ,表明碱诱导蛋白质分子变性使蛋白质分子充分伸展 ,将更多疏水性氨基酸残基暴露于溶剂中。更多还原

【Abstract】 The microenvironment change of aromatic amino acid residues in BSA at various pH were studied by fluorescence spectra and ultraviolet absorption spectra.It suggested that the change of protein surface and surface hydrophobicity from result.Compared to neutral pH,the Trp microenvironment seemed to be more hydrophobic at lower pH indicating less local surface hydrophobicity of BSA.At higher pH,the protein undergo conformational changes and undold,the more buried Phe exposed to solvents consistent with stronger surface hydrophobicity of BSA.更多还原