【摘要】 研究了在pH 6 2～ 8 2磷酸二氢钾 磷酸氢二钠缓冲体系中 ,非离子表面活性剂TritonX 10 0存在下 ,铜 (Ⅱ )与二溴羟基苯基荧光酮 (DBH PF)形成 1∶2红色三元混配合物而使荧光熄灭 ,建立了荧光熄灭法测定铜的新方法。配合物的λex=5 3 0nm ,λem=5 60nm ,含量在 0～ 80 μg·L-1范围内有良好的线性关系 ,检测限为 0 2 μg·L-1。体系的灵敏度高 ,选择性好。Al(Ⅲ )、Fe(Ⅲ )、Ti(Ⅳ )的干扰 ,可用氟化钠、苦杏仁酸掩蔽。方法快速简便 ,用于人发、茶叶及水中微量铜的测定与AAS相符 ,RSD(n=5 ) <6 9% ,回收率在 98%～ 10 4 %之间。更多还原

【Abstract】 A fluorescence quenching method for the determination of trace Cu(Ⅱ) was developed.The method was based on the fluorescence quenching effect of DBH PF and Triton X 100 due to the formation of a non fluorescent ternary complex in pH 6 2-8 2 KH 2PO 4 Na 2HPO 4 medium. The excitation and emission wavelengths are 530 and 560 nm,respectively.The linear range is 0-80 μg·L -1 and the detection limit is 0 2 0 μg·L -1 for Cu(Ⅱ).The method has been used to determine trace amounts of Cu(Ⅱ) in hair,tea and water.The recovery is 98%-104%,and the relative standard deviation is less than 6 9% with satisfactory results.更多还原