【摘要】 从广东省南澳县西山等养蛙场多次收集患黄杆菌病牛蛙,其症状是:歪头、狂游、双眼白内障和腹水等。从病蛙脑浆、眼底和腹水中分离到多株病原菌,急性毒性试验表明病原菌对健康牛蛙致病力强。取有代表性的981007-2等菌株进行系统的生理生化试验显示,革兰氏染色阴性、无鞭毛、无芽孢的短杆菌;分离菌株内毒素检验阳性;病原菌显示了对青霉素类药物高度耐药、好氧型、利用葡萄糖等生理、生化特性。经VITEK-AMS-60自动化检测系统鉴定为黄杆菌IIb群(Plavobacterium group IIb)。选用47种抗菌药物,通过K-B纸片扩散法对病原菌进行耐药性研究结果,分离菌株对38种抗菌药物显示耐药(占80.85％)、对4种药物中等敏感(占8.51％),仅对利福平、环丙沙星、氧氟沙星、氟啶酸、麦迪霉素等5种药物(占10.64％)敏感。应用试管稀释法测定上述5种敏感药物的平均MIC分别是0.19、0.27、0.53、2.40和10.2μg/ml。更多还原

【Abstract】 The xick Rana catesbianaes were collected from Xishan culture farm and other places, in Nanao prefecture, Guangdong province. The symptoms of Flavobactrium diseases include askew heads, swimming madly, cataract in both eyes, livers congestion and abdominal dropsy ect. The strains of pathogens were isolated from the Rana catesbianaes’brains, eye grounds and ascitic fluid. The acute toxicity tests proved the isolated strains to be highly pathogenic to Rana catesbiana. Sytematic physiological and biochemical tests of three representative strains (including strain 981007-2) showed that the isolated pathogens were short-rod-shaped with rounded ends, no flagellum, no endospores, Gram-negative, aerobic, highly resistant to Penicillum agents, positive in endotoxin test and glucose utilizable. The pathogens were indentified to be Flavobacterium group IIb by VITEK-AMS-60 automatic detecting system. The studies on drug resistance of pathogens to 47 antimicrobial agents by the K-B paper disk method showed that 68 tested antimicrobial agents (80.85%) were resistant, 4(8.51%) intermediate, 5(10.64%) susceptible. By the method of test tube dilution, the drug resistance of the pathogens were tested with the 5 susceptible agents above. The MIC of them were rifampicin 0.19, ciprofloxacin 0.27, ofloxacin 0.53, enoxacin 2.40 and midecamycin 10.2μg/ml.更多还原