【摘要】 从人胎脑cDNA文库中克隆到一条长 3 479bp的cDNA ,它含一个长 90 3bp的开放阅读框架 ,拟编码一个 30 0个氨基酸的蛋白质 ,其分子质量为 35 397u ,等电点为 5 .35 ,与酵母MAK16蛋白的同源性为 41% .该编码蛋白有一个双侧核定位信号motif和一个RNA结合motif,将这一新cDNA序列推导的蛋白命名为RNA结合基序蛋白 13 (RBM 13) ,基因定名为RBM13 .用RBM 13基因的cDNA探针进行Northern杂交 ,检测到 3 6kb和 2 2kb二种长度的转录本 ,在心脏、骨骼肌、肾脏和肝脏中有较高表达 .更多还原

【Abstract】 A full length cDNA with 3 479 bp was cloned from a fetal brain cDNA library. It contains an open reading frame encoding a 300 amino acid protein with a calculated molecular mass of 35 379 u and a pI of 5.35. The deduced protein, which was named as RNA binding motif protein 13 (RBM13), shows 41 % identity to MAK16 protein of S. cerevisiae . It contained the signals of a bipartite nuclear localization and an eukaryotic putative RNA binding region RNP 1. Northern blot analysis showed that two sizes of human RBM 13 messenger RNAs (3.6 kb and 2.2 kb) were widely appeared, with highest levels in heart, skeletal muscle, kidney and liver.更多还原