【摘要】 质粒 pGA46 4带有从谷氨酸棒杆菌染色体上分离到的有启动功能的片段 ,用PCR技术从 pGA46 4中扩增了该片段的关键区域 :启动子PGL.将该启动子经EcoRⅠ BamHⅠ双酶切后 ,与大肠杆菌质粒 pJL0 1的EcoRⅠ BamHⅠ大片段连接 ,再接入XylEgene和棒状杆菌质粒pXZ 10 142 ,构建成棒状杆菌 大肠杆菌穿梭表达载体 pJL2 3.用邻苯二酚双加氧酶基因检测表明 ,该表达载体可在棒状杆菌中高效地表达外源基因 .更多还原

【Abstract】 Plasmid pGA46 4 contains a promoter function fragment isolated from the chromosome of Corynebacterium glutamicum . The essential part of this fragment promoter P GL is amplified from pGA46 4 by PCR. In order to construct a shuttle expression plasmid pJL23, this promoter was ligated with Escherichia coli plasmid pJL01, the Xyl E gene and the corynebacteria plasmid pXZ10142. According to the Catechol 2,3 dioxygenase expression results, the expression vector functions well in the expression of foreign genes in corynebacteria.更多还原