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五指山小型猪同期发情和超数排卵方法的探讨
Study on Synchronization and Superovulation In Miniature Pig
【摘要】 本试验用五指山小型猪进行同期发情和超数排卵技术的试验研究.将 2 5头小型猪母猪随机分为三组,每一组各采用一种超排处理方法,即Ⅰ组为PMSG +HCG法,Ⅱ组为PG/PMSG +HCG法,Ⅲ组为PG/PG +PMSG +HCG法.三种处理方法的同期发情率和超排有效率分别为:Ⅰ组 6 6 7%和 50 0 %,Ⅱ组 90 2 %和 6 6 7%,Ⅲ组为 10 0 %和 85 6 %.试验同时采用手术法观察母猪卵巢的黄体数做为确定超排成功与否的依据,并用输卵管冲卵法收集胚胎.三种处理方法获得胚胎检出率分别为:Ⅰ组 2 6 87%,Ⅱ组 50 76 %,Ⅲ组6 5 85%.试验结果表明,PG/PMSG +HCG组和PG/PG +PMSG +HCG组的超排效果显著好于PMSG +HCG处理组(P <0 0 1,P <0 0 5)
【Abstract】 Wu Zhi Shan miniture pigs were used for synchronization and Superovulation. A total of 25 miniature pigs were divided into three groups in different methods: Ⅰ:PMSG+HCG;Ⅱ:PG/PMSG+HCG; Ⅲ:PG/PG+PMSG+HCG. The rate of synchronization and the effect rate of superovulation in three groups were (Ⅰ)66 7%,50 0%;(Ⅱ)90 2%,66 7%; (Ⅲ)100%,85 6%; respectively. The effect of superovulation was identified by counting of corpus luteum on the ovary and flushing embryos from oviducts. The recovery rate of embryos in three groups were 26 87%, 50 76% and 65 85% respectively. The results indicated that treatment Ⅱand Ⅲ (PG/PMSG+HCG; PG/PG+PMSG+HCG) was better than tretment Ⅰ(PMSG+HCG)(P<0 01).
- 【文献出处】 北京农学院学报 ,JOURNAL OF BEIJING AGRICULTURAL COLLEGE , 编辑部邮箱 ,2000年01期
- 【分类号】S828.1
- 【被引频次】15
- 【下载频次】246