【摘要】 利用电化学方法研究了黄芪、绞股蓝及其制剂对O₂^■-（邻苯三酚自氧化产生）和·OH（Fenton体系产生）的清除作用。结果表明:黄芪和绞股蓝及其制剂均能有效地清除活性氧自由基,但对不同自由基的清除效能不同,其中人参皂甙Rb₁和黄芪甲甙对O₂^-和·OH的抑制作用最强。黄芪样品对O₂^-的清除作用能力依次为:黄芪甲甙>生黄芪>内蒙黄芪>河北黄芪>黄芪对照品>蜜炙黄芪;对·OH的清除效能分别为:黄芪甲甙>河北黄芪>蜜炙黄芪>黄芪对照品>生黄芪>内蒙黄芪。绞股蓝样品对O₂^-清除能力的顺序为:人参皂甙Rb₁>绞股蓝双花茶>绞股蓝总甙片>安国药市绞股蓝>河北青县绞股蓝;而对·OH的抑制效能则为:人参皂甙Rb₁>绞股蓝双花茶>安国药市绞股蓝>河北青县绞股蓝>绞股蓝总甙片。更多还原

【Abstract】 The scavenging effects of Astragalus and Gynostemma pentaphyllum with its products on O2T (produced by the autoxidation of pyrogallol) and ·OH (generated from the Fenton reaction) were investigated by electrochemical method. The results showed that they could scavenge the active oxygen free radicals effectively, however, their scavenge ability was different for O2T and ·OH. The inhibitory effect of Ginsenoside Rb1 and Astragaloside Ⅳ on O2T and·OH was the most strongest. Their IC50 values were calculated respectively. The scavenging ability of Astragalus samples on O2T was in the order of the following: astraga-loside Ⅳ > crude radix astragali > mongolia radix astragali > hebei radix astragali > radix astragali > radix astragali mixfry with honey; and the scavenging effect on ·OH was: astragaloside Ⅳ > hebei radix astragali > radix astragali mix-fry with honey > radix astragali > crude radix astragali > mongolia radix astragali. The scavenging activity of gynostemma samples on O2T was: ginsenoside Rb1 > gynostemma jujube tea> tabellae Jiaogulanosidi > anguo market gynostemma > hebei qingxian gynostemma; while on · OH, the inhibitory ability was: ginsenoside Rb1 > gynostemma jujube tea > anguo market gynostemma > hebei qingxian gynostemma> tabellae Jiaogulanosidi.更多还原