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薏苡属(CoixL.)2种1变种的遗传多样性及亲缘关系的初步分析

Analysis of genetic diversity and taxonomic relations among two species and one varity of \%Coix \%L.

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【作者】 马建霞; 潘泽惠; 刘心恬; 於虹;

【Author】 Ma Jianxia, Pan Zehui, Liu Xintian, Yu Hong (Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014),

【机构】 江苏省中国科学院植物研究所!南京210014;

【摘要】 利用垂直板型聚丙烯酰胺凝胶电泳技术,检测了薏苡属(CoixL.)2种1变种:薏苡(CoixlacrymajobiL.)6个居群、念珠薏苡(C.lacrymajobivar.maximaMakino)3个居群和薏米(C.chinensisTod.)2个居群共11个居群5个酶系统6个位点上9个等位基因的分布情况,结果表明:薏苡、念珠薏苡和薏米的遗传多样性水平普遍较低,每个位点等位基因平均数(A)分别为12,11和12;多态位点百分数(P)分别为167,113和167;平均期望杂合度(He)分别为006,003和007。聚类分析不能从遗传上将以上的2种1变种区分开。

【Abstract】 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholicJ. Plant Resour. & Environ. 1999, 8(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholic& Environ. 1999, 8(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholicEnviron. 1999, 8(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholic1999, 8(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholic8(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholic(4): 11~16 Allozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholicAllozyme variation at 6 loci encoding 5 enzymes was examined in 6, 3 and 2 populations across the ranges of \%Coix lacryma\|jobi \%L., \%C. lacryma\|Jobi \%var. \%maxima \%Makino and \%C. chinensis \%Tod., respectively, using vertical slab polyacrymide gel electrophoresis. The result indicated that all three species are genetically depauperate in their population: mean number of alleles per locus (A) is 1.2, 1.1 and 1.2; percentage of loci polymopholic(P) is 16.7,11.3 and 16.7;mean expected heterozygositymean expected heterozygosity(He) is 0.06, 0.03 and 0.07, respectively. Cluster analysis indicated that three species can not be distinguished each other conspicuously.

【关键词】 等位酶; 薏苡属; 居群; 遗传多样性;
【Key words】 allozyme; \%Coix \%L.; population; genetic diversity;
【基金】 国家自然科学基金资助项目 !(编号 :3 9670 0 87)
  • 【文献出处】 植物资源与环境 ,Journal of Plant Resources and Environment , 编辑部邮箱 ,1999年04期
  • 【分类号】Q943
  • 【被引频次】11
  • 【下载频次】126
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