【摘要】 探讨亚硒酸钠和维生素Ｅ对香烟烟雾冷凝物（ＣｉｇａｒｅｔｅＳｍｏｋｅＣｏｎｄｅｎｓａｔｅ，ＣＳＣ）诱发的ＤＮＡ损伤的保护／对抗作用。采用非程序ＤＮＡ合成（ＵｎｓｃｈｅｄｕｌｅｄＤＮＡＳｙｎｔｈｅｓｉｓ，ＵＤＳ）试验，对两种ＣＳＣ诱发ＤＮＡ损伤和Ｓｅ、ＶＥ抗ＣＳＣ的致突变作用进行了观察。结果表明，在实验浓度范围内，两种ＣＳＣ均可诱发大鼠肝细胞ＤＮＡ损伤，与空白对照相比，差别有高度显著性（Ｐ＜００１），Ｓｅ和ＶＥ均能抑制ＣＳＣ的诱变性，抑制率在１４４７％～３７０２％，有明显的剂量反应关系（ｒＳｅ＝０９０７５，ｒｖｃ＝０９０８８，Ｐ＜００５）。当Ｓｅ和ＶＥ联合作用时，抑制率达４２９８％，呈现出协同抗诱变效应。结论：Ｓｅ和ＶＥ具有不同程度的抗ＣＳＣ的致突变作用，两种抗诱变剂的同时使用是合理的抗诱变／抗癌预防手段。更多还原

【Abstract】 To study effects of protection and antagonism of sodium selenite and vitamin E antagonizing DNA damage caused by Cigarette Smoke Condensate(CSC).Methods Unscheduled DNA Synthesis (UDS) assay was used.DNA damage induced by two CSC was studied.In the meantime,effect of sodium selenite and vitamin E antagonizing mutagenesis caused by CSC were observed.Results In the range of test concentration,two CSC induced DNA damage of liver cell of rats,there is significance(P<0 01) as compared with blank control.Sodium selenite and vitamin E suppressed mutagenicity of CSC.Inhibition rate is from 14 47%～37 02%,in a manner of dose response relationship (r Se =0 9075,r VE =0 9088,P<0 05).If use of Se and V E combined,inhibition rate reached 42 98%.There is anti mutagenic effect of incorporation.Conclusion Se and V E have effets of antagonizing mutagenesis in varying degrees.The combined use of sodium selenite and Vitamin E is a rational means of prevention for mutagenesis and cancer.更多还原