【摘要】 目的　探讨肝纤维化形成不同阶段ＴＧＦβ１和Ⅰ、Ⅲ型胶原表达的变化规律及其相互关系。方法　建立肝纤维化动物模型；分别用免疫组化法和斑点杂交法检测肝纤维化不同阶段ＴＧＦβ１、Ⅰ、Ⅲ型胶原蛋白及其ｍＲＮＡ表达。结果随着肝纤维化程度的加重，ＴＧＦβ１、Ⅰ、Ⅲ型胶原蛋白及其ｍＲＮＡ表达量都逐渐增高，各实验组与对照组相比，其差异均有显著性（Ｐ＜０．０５）。结论肝纤维化时，肝内ＴＧＦβ１、Ⅰ、Ⅲ型胶原蛋白的表达呈正相关，提示ＴＧＦβ１是重要的肝纤维化介质。更多还原

【Abstract】 Objective To study the correlation between expression of TGFβ1, and type Ⅰ and Ⅲ collagen in normal and CCl4. induced fibrotic rat lived Methods An animal model of hepatic fibrosis was established by subcutaneous injection of 50% CCl4. .The content of TGFβ1,, type Ⅰ and Ⅲ collagen proteins and the expression of TGFβ1,, α1, (Ⅰ) and a, (Ⅲ) procollagen mRNA in the different stage of hepatic fibrogenesis was detected by immunohistochemical staining and Dot blot method, respectively. Results During the progression of hepatic fibrosis, the expression of TGFgβ1, type Ⅰ andⅢ collagens gradually increased.The intensity of expression in the experimental group was greater than those in the control groups (P<0.05). Conclusion The expression of TGFβ1 is positively correlated with that of type Ⅰ and Ⅲ collagen,suggesting its important role in the pathogenesis of liver fibrosis.更多还原