【摘要】 建立应用13/21染色体特异性探针荧光原位杂交技术(FISH)检测种植前人类胚胎13/21染色体非整倍体的方法。方法:应用FITC标记的13/21染色体着丝粒部位杂交的α卫星重复序列探针对27个未受精卵母细胞和36个多精受精胚胎的卵裂球进行原位杂交。结果:单倍体卵母细胞核呈现2个杂交信号,固定率80%,杂交效率67%;多精受精胚胎的卵裂球核呈现6个杂交信号,固定率72%,杂交效率65%。结论:应用荧光原位杂交技术在卵母细胞和胚胎细胞可检测13/21染色体非整倍体。更多还原

【Abstract】 Objective: To study the aneuploidy of chromosome 13 and 21 in blastomeres developed in vitro. Methods: Using alpha satellite repetitive probe specific for chromosome 13 and 21, hybridizing 27 infertilization ovum and 36 blastomeres from polyspermic embryos for inter FISH. Results: Ovum produced 2 hybridization signals, fixing rate was 80%, hybridization rate was 67%; Blastomeres produced 6 hybridization signals, fixing rate was 72%,hybriditagion rate was 65%. Conclusions: FISH can detect chromosome aneuploidy effectively in ovnm and blastomeres and can be applied to preimplantation genetic diagnosis.更多还原