【摘要】 经非猪瘟免疫猪增殖猪瘟病毒石门株，从抗凝血中直接提取病毒ＲＮＡ进行ＲＴ－ＰＣＲ，获得了猪瘟病毒石门株Ｅ２基因片段。克隆后测序，结果表明石门株的Ｅ２囊膜糖蛋白与国外报道的５株猪瘟病毒的Ｅ２囊膜糖蛋白具有相同的骨架结构，即均具有１５个半胱氨酸残基和５个潜在糖基化位点，此外石门株也具有保守序列ＲＹＬＡＳＬＨ。同源性比较表明，石门株与日本的ＡＬＤ株和ＧＰＥ－株同源性最高，而与欧洲的Ａｌｆｏｒｔ株同源性最低。与国内的疫苗种毒（４８２代）、疫苗毒以及国外使用的中国猪瘟兔化弱毒株同源性比较表明，石门株与种毒（４８２代）同源性最高，其次为国外使用的中国猪瘟兔化弱毒株，而与国内使用的疫苗毒同源性最低。本研究提示国内疫苗毒的Ｅ２基因在生产中有较大变异，可能导致抗原漂移。更多还原

【Abstract】 E2 gene of CSFV shimen strain was amplified by RT-PCR method from the blood of infected pig, then cloned and sequenced. Results show that the glycoprotein E2 of Shimen strain has the same number and locations of cysteine residues(15)and potential N linked glycosylation sites(5)with that of the previously reported five CSFV strains. It also has conserved motif RYLASLH. E2 nucleotide sequence and amino acid sequence homolo gy comparison show that the shimen strain has significant homology with Japanese strains (ALD and GPE~-), but lower homology with Alfort strain. In addition, it has significant homology with Chinese stock vaccine strain, and lower homology with Chinese vaccine virus. These results suggest that E2 gene of Chinese vaccine virus mutates rapidly during production and may cause antigenic drift.更多还原