【摘要】 目的：家蝇对有机磷及氨基甲酸酯类的抗药性与家蝇的酯酶活性密切相关，采用滤纸法快速检测野外品系家蝇的酯酶活性，分析家蝇抗药性的发生发展规律，探讨早期预测及防治策略。方法：提取雌性家蝇头部的酯酶蛋白，采用α醋酸萘酯作用，固兰盐染色测定滤纸的吸光值，分析酯酶活性。结果：家蝇对有机磷杀虫剂的抗药性与αＮａ酯酶的活力呈极度相关性（ｒ＝０９３９０，Ｐ＜０００５），氨基甲酸酯类杀虫剂的抗药性与αＮａ酯酶的活力呈显著相关性（ｒ＝０８１９７，Ｐ＜００５），拟除虫菊酯类杀虫剂的抗药性与αＮａ酯酶的活力不相关（ｒ＝０７４０６，Ｐ＞００５）。结论：酯酶滤纸法技术应用于家蝇抗性早期诊断及抗药性发生机理研究颇具意义更多还原

【Abstract】 In order to rapidly detect the resistant level of housefly, to organophosphate or carbanate insectides. The individual esterase activity was measured by filter paper test. The results showed that the esterase activity increased in the DDVP or fenobcarb resistant strains but didn’t raise in deltamethrin resistant strains. There were positive relativity between esterase activity and resistance ratio to DDVP or fenobcarb. The inhibitory frequency of DDVP or fenobcarb to esterase activity in the susceptible population was evidently higher than that of the DDVP or fenobcarb resistant strains.更多还原