Inhibiting effects on HeLa cell growth by HER-2 gene targeting

【摘要】 <正> A plasmid (promoter pPNT 1305) for HER-2 gene targeting was constructed and introduced me HeLa cells by electroporation . After positive selection by G418 and negative selection by Gancyclovir, the targeted cell line (HER-2-1 cells) containing only one copy of HER-2 allies was selected. Northern blot assay showed that the mR-NA expression of HER-2 was significantly less than that of its parental cells. The results of Western blot also showed that HER-2 protein of HER-2 - cells was obviously less than that of HeLa cells. The dala of FA.CS assay demenstrated that after one of the two HER-2 copies in HeLa cells was knocked out, the G1 phase percentage of HER-2 cells was more and the M plus G2 phase percentage of HER-2 -1 cells was less than those of HeLa ceils respectively These reaults show that gene targeting of HER-2 has exhibited the inhibiting effects on the proliferational capability of HeLa cells更多还原

【Abstract】 A plasmid (promoter pPNT 1305) for HER-2 gene targeting was constructed and introduced me HeLa cells by electroporation . After positive selection by G418 and negative selection by Gancyclovir, the targeted cell line (HER-2-1 cells) containing only one copy of HER-2 allies was selected. Northern blot assay showed that the mR-NA expression of HER-2 was significantly less than that of its parental cells. The results of Western blot also showed that HER-2 protein of HER-2 - cells was obviously less than that of HeLa cells. The dala of FA.CS assay demenstrated that after one of the two HER-2 copies in HeLa cells was knocked out, the G1 phase percentage of HER-2 cells was more and the M plus G2 phase percentage of HER-2 -1 cells was less than those of HeLa ceils respectively These reaults show that gene targeting of HER-2 has exhibited the inhibiting effects on the proliferational capability of HeLa cells更多还原