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利用花椰菜无菌苗顶芽进行试管繁殖研究
A study on rapid in vitro propagation of cauliflower seedlings with germ free apical buds
【摘要】 本研究以花椰菜无菌苗顶芽为外植体, 诱导出不定芽, 进行了快速繁殖, 并对影响试管苗素质和繁殖系数的因素作了探讨。试管苗在 M S- 199 培养基上,每代每月繁殖系数达5 倍多,在无激素的 M S培养基上生根率达到98% 以上, 批量移栽平均成活率达到98% , 再生植株在田间正常结球, 开花结籽, 具有良好的遗传稳定性。
【Abstract】 In the present study, cauliflower seedlings with germ free apical buds were taken as explants, from which adventitious buds were induced and rapidly propagated; and some factors affecting in vitro seedling quality and reproductive coefficient were probed into. The reproductive coefficient of the seedlings in vitro on MS 199 medium reached to 5 times per generation in 1 month. The strike root rate of the seedlings on hormone free MS medium reached to more than 98%, the average survival rate of the seedlings reached to 98% under conditions of batch transplantation. Regenerated plants could normally blossom and fruit bear with stable characteristics of genetics in fields.
【Key words】 cauliflower; germ free apical bud; isolated culture; adventitious bud; enrichment culture;
- 【文献出处】 浙江农业科学 ,JOURNAL OF AGRICULTURAL SCIENCE , 编辑部邮箱 ,1999年05期
- 【分类号】S635.303
- 【被引频次】1
- 【下载频次】52