【摘要】 目的　以流式细胞术法简便、有效地检测凋亡和坏死细胞。方法　将热处理、喜树碱诱导和紫外线诱导的急性早幼粒白血病细胞株（ＨＬ６０） 细胞分别用碘化丙锭（ＰＩ） 摄入试验、亚Ｇ１ 峰法和ＤＮＡ 链缺口标记法（ＴｄＴ） 进行检测，并与对照组细胞进行比较，观察三种方法在凋亡和坏死细胞检测中的应用情况。结果　ＰＩ摄入试验显示，死细胞可大量摄取染料，ＤＮＡ 含量直方图上其相对荧光强度明显较活细胞高；凋亡细胞在经磷酸柠檬酸盐缓冲液（ＰＣ） 处理后，Ｇ１ 期峰前出现一额外亚Ｇ１峰而被识别，未经ＰＣ 处理的细胞难检测到凋亡；ＴｄＴ 标记时，坏死细胞平均荧光强度约为对照的３倍，而凋亡细胞荧光强度明显增加。结论　将ＰＩ摄入试验、亚Ｇ１ 峰法和ＴｄＴ 法结合起来，可有效检测凋亡和坏死细胞。更多还原

【Abstract】 Objective To detect necrotic and apoptotic cells effectively and simply by flow cytometry.Methods Necrotic or apoptotic HL 60 cells which were induced by thermodynamics, camptothecin and ultraviolet radiation were analyzed with propidium iodide(PI) absorption test,Sub G1 and TdT method. The effect of the methods were compared.Results Death cells absorbed a mass of PI. The relative fluorescence intensity was higher in death cells than in live cells on DNA histogram. Apoptotic cells treated with phosphate citric acid buffer (PC) were distinguished by extra Sub G1 peak ahead of G1 peak on DNA histogram, but it was difficult to detect apoptosis for those not treated with PC. After labelled with TdT method, the mean fluorescence intensity of necrotic cells was 3 fold of the control, meanwhile, the mean fluorescence intensity of apoptotic cells increased apparently.Conclusion We can detect necrotic and apoptotic cells efficiently by the method of PI uptake,Sub G1 and TdT.更多还原