【摘要】 目的　探讨细胞间粘附分子 1(ICAM 1)在恶性黑素瘤细胞株的表达及临床意义。方法　用体外细胞培养方法、逆转录聚合酶链反应技术 ,观察恶性黑素瘤细胞株MM 8 1在温热及某些细胞因子刺激下 ,细胞形态、细胞增殖能力及ICAM 1的基因水平变化。结果　温热、γ 干扰素、肿瘤坏死因子α可使细胞形态改变、细胞增殖能力下降、ICAM 1的表达增加 ,白介素 2未能影响细胞形态及增殖能力 ,也未能使ICAM 1明显改变。结论　恶性黑素瘤细胞株ICAM 1的表达与细胞被破坏有关 ,同时为温热、γ 干扰素、肿瘤坏死因子 α作为治疗恶性黑素瘤的辅助手段提供了理论依据。更多还原

【Abstract】 Objective To investigate the expression of ICAM 1 in human malignant melanoma (MM)cell lines and its clinical significance.Methods Human MM cell lines MM8 1 were cultured and analyzed by reverse transcription polymerase chain reaction(RT PCR), the cell shape,cell growth ability and ICAM 1 by mRNA expression under the stimulation of hyperthermia or cytokines were observed.Results Hyperthermia、γ IFN and TNF αcould change cell shape,reduce cell growth ability and increase the expression of ICAM 1.IL 2 could not influence the cell shape and the expression of ICAM 1.Conclusion The expression of ICAM 1 in MM cell lines may be correlated with the cell destruction,which provides theoretical basis of supplementary therapy for MM with hyperthermia、IFNγand TNFα.更多还原