【摘要】 目的研究胰淀素对磺脲类降糖药刺激大鼠胰岛细胞分泌胰岛素作用的影响和机制。方法用放免和荧光方法分别检测经不同浓度胰淀素温育后再行优降糖刺激试验的胰岛细胞胰岛素分泌量、细胞内Ｃａ２＋含量。结果在胰淀素５μｍｏｌ／Ｌ、１０μｍｏｌ／Ｌ组中，３ｎｍｏｌ／Ｌ优降糖刺激下的胰岛素分泌量分别为（３８±１０）μｇ／Ｌ、（２１±１０）μｇ／Ｌ，均比对照组（４９±０９）μｇ／Ｌ低（ｔ＝２３１３，Ｐ＜００５；ｔ＝５８８７，Ｐ＜００１）；细胞内Ｃａ２＋含量为（２７０±１５）ｎｍｏｌ／Ｌ、（１３０±１５）ｎｍｏｌ／Ｌ，均显著低于对照组（３３０±１８）ｎｍｏｌ／Ｌ，并呈剂量相关性（ｔ＝７２４３，Ｐ＜００１；ｔ＝２４１４３，Ｐ＜００１）。结论高浓度的胰淀素抑制优降糖刺激胰岛β细胞内Ｃａ２＋浓度增加可能是胰岛素分泌减少的机制之一。更多还原

【Abstract】 Objective To study the effect and mechanism of amylin upon oral sulfonylurea drugs and its action to stimulate the islet cell secretion of insulin in rats. Methods Radioimmunogical and fluorescence methods were used to determine the amount of insulin secretion as well as the content of intracellular Ca 2+ of the rat islets incubated with amylin in different concentrations and followed by glyburide stimulation test. Results In amylin concentrations of 5 μmol/L and 10 μmol/L, 3 nmol/L glyburide decreased insulin release volume, being (3.8±1 0) μg/L and (2.1±1.0) μg/L as compare with (4.9±0.9) μg/L of the control group ( t =2.313, P <0 05; t =5.887, P <0.01). Intracellular Ca 2+ content in the two concentrations was (270±15) nmol/L and (130±15) nmol/L, being markedly decreased as compared with (330±18) nmol/L of the control group. The changes were dosage related ( t =7.243, P <0.01; t =24.143, P <0.01). Conclusion High concentration of amylin is resposible for the prevention of the rise of Ca 2+ inside in the islet β cell acted upon by sulfonylurea drugs, this may be one of the mechanisms that bring about the decreased release of insulin.更多还原