【摘要】 目的：明确鼻咽癌（NPC）细胞中小鼠双微粒体基因（MDM2）的扩增与表达情况及其作用。方法：应用斑点杂交、半定量逆转录一多聚酶链反应技术、原位杂交、免疫组化分别对NPC、慢性鼻咽炎症粘膜CINE）进行MDM2基因扩增与表达的检测；运用免疫组化、PCR分别对NPC、CINE中p53表达、PCNA和Ki67指数、EB病毒DNA进行检测；同时分析MDMZ的过表达与p53表达、PCNA和Ki67指数、EB病毒潜伏感染、NPC各临床病理参数的关系。结果：3．1％的NPC有MDM2基因的扩增，30.8％有MDM2mRNA高表达，30.4％有MDM2蛋白的过表达；MDM2的过表达与p53表达（p＜0.05）、NPC颈淋巴结转移相关（p＜O．05），而与EBV潜伏感染，PCNA和Ki67指数、NPC的T分期、NPC病人的年龄、性别、EBVCA-IgA滴度无关。结论：MDM2基因在NPC组织中的主要活化方式是过表达而不是扩增；MDM2基因可能通过与p53相互作用而NPC的发生和转移过程中起着重要作用。更多还原

【Abstract】 To detect the status and potential role of MDM2 gene in nasopharyngeal carcinoma (NPC). Methods: MDM2 gene amP1ification and overexpression in NPC and chronic inflamation of nasopharyngeal epithelium (CINE ) were determined by DNA dot blot, semi-quantitation reverse transcription-polymerase chai n react ion (RT- PCR ), in si tu hybridization (ISH ),and immunohistochemistry (IHC ) separately. p53 protein, PCNA and Ki67index, and EB virus DNA were also detected by IHC and PCR separately. The re1ationship between MDM2 gene overexpression and p53 protein expression, EB virus latent infection, cell proliferation index and clinical pathological parameters were analyzed. Results: About 3. 1 % NPC samples showed MDM2 gene amplification, ahaut 3O. 8 % MDM2 mRNA overexpression, and 3O. 4% MDM2protein positive. The MDM2 gene overexpression in NPC was significantly related to p53 protein expression level (P< O. O5) and cervical lymPh nocle metastasis(P<0. O5 ), but not related to EB virus latent infection, Ki67 and PCNA index,patient’s age, sex, EBVCA-IgA titre and T stage. Conclusion: MDM2 gene may play an important role in the pathogenesis and process of metastasis of NPC through interaction with p53 gene.更多还原