【摘要】 目的探讨Ｔ－２毒素与ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ的双重作用对小鼠肝脏脂质过氧化物的影响。方法实验采用析因设计，ＢＡＬＢ／Ｃ小鼠随机分成４组，Ｉ正常对照组；Ⅱ病毒组；ⅢＴ－２毒素组；Ⅳ病毒＋毒素组。病毒组常规腹腔接种０．１ｍｌ内含１０００ＴＣＩＤ５０的１６４０营养液；毒素组隔日按１．０ｍｇ／ｋｇ体重Ｔ－２毒素灌胃，直至实验结束；病毒＋毒素组先Ｔ－２灌胃，３周后腹腔接种同稀释度病毒。各组鼠于病毒接种后第７天，１４天，２１天，３０天，５０天分批处死测定肝脏脂质过氧化物（ＬＰＯ）含量。结果显示病毒和Ｔ－２毒素均可非常明显（Ｐ＜０．０１）引起小鼠肝脏ＬＰＯ含量升高。两者双重作用更为明显，但两者之间没有交互作用。结论ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ和Ｔ－２毒素作用于生物体可导致机体脂质过氧化损伤，其效应强于单纯给予Ｔ－２毒素或ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ感染更多还原

【Abstract】 Objective To research the combined effect of Coxsackievirus B 3m and T 2 toxin on the metabolism of lipid peroxide by factorial design.Methods BALB/C mice as experimental animals were randomly divided into 4 groups:1.control group;2.virus group;3.T 2 toxin group;4.virus and T 2 toxin group.The mice in group 2 were injected with Coxsackievirus B 3m liquor 1000 TCID 50 in the dose of 0.1ml into abdominal cavity.The mice in group 3 were forced to swallow T 2 toxin every other day in the dose of 1.0mg/kg until the end of the experiment.The mice in group 4 were treated with T 2 toxin in the same way in the first 3 weeks,then the same concentration viral liquor like that used in group 2 was inoculated.The content of LPO in 4 groups was determined when the mice were killed in the seventh,fourteenth,twentieth,thirtieth and fiftieth day after inoculation.Results The results show that both virus and T 2 toxin have significant influence on the content of LPO in mice liver.The interaction between virus and T 2 toxin was not remarkable.Conclusions The obvious lipid peroxidation induced by coxsackievirus B 3m and T 2 toxin is more serious than that caused by Coxsackievirus B 3m or T 2 toxin respectively.更多还原