【摘要】 通过对分离自洗煤厂的一株青霉菌进行人工诱变，选育出一株具有较强降解褐煤能力的突变株．将褐煤加到平板培养的该菌株上，经过３６ ｈ 开始被降解．将该菌株用摇瓶培养３ ｄ ，其培养液也能降解褐煤．元素分析结果表明，降解物中 Ｈ、 Ｏ、 Ｎ 的元素质量分数（ ｗ／ ％ ） 比原煤均有所升高，而ｗ（ Ｃ）／ ％ 明显下降．降解产物的经验分子式可写为 Ｃ１００ Ｈ１４４ Ｏ５８ ．２ Ｎ１０ ．０ Ｓ１ ．１８ ，经凝胶过滤法测，定其 Ｍｒ 分布在（２ ～８） ×１０４ 之间； 其红外吸收光谱与原煤相比也有一定的变化． 这些结果说明生物降解过程中褐煤的组成和结构发生了一定的变化更多还原

【Abstract】 A strong lignite degrading mutant was selected by UV treatment of Penicillium sp. which was isolated from coalmine slurry. The mutant could start to degrade lignite from 36 h after adding lignite onto the mycellium incubated in plate, and the filtrate of the liquid culture incubated for 3 d in shaking condition could also degrade lignite. Elemental analysis indicated that compared with the original lignite, the composition of the degraded products changed. The percents of H, O, and N contents ( w /%) were increased, but the percent w (C)/% content was decreased. According to the principle of the simplest molecular formula, the molecular formula of the bioproduct could be written as C 100 H 144 O 58.2 N 10.0 S 1.18 . The M r of the bioproduct was estimated between (2～8)×10 4. Its IR spectra were also different from the original lignite.更多还原