【摘要】 采用小鼠原代成纤维细胞作为饲养层，在含1×103单位白血病抑制因子（LIF）的DMEM高糖培养基中，建成了11个C57BL／6J小鼠的ES细胞系，成系率为9．6％。所建的11个ES细胞系中有5个核型正常率大于70％。这些细胞具早期胚胎细胞的特征，呈碱性磷酸酶阳性，具表达0014基因的特性5进行体内分化实验时能发生广泛的分化。通过嵌合体制作实验证明其中3个系具嵌合能力，并从中筛选出具高效种系嵌合能力的MESPU35细胞系，MESPU35细胞的克隆能达到种系传递，经过基因操作的细胞克隆，也保留了高效的嵌合能力。因此，MESPU35细胞可作为制作突变小鼠的有效载体。更多还原

【Abstract】 Eleven ES cell lines from C57BL/ 6J mice were established, with the primaryculture of mice embryos as feeder cells and 1×103 units Leukemia Inhibitory Factor(LIF) in the DMEM (high glucose) medium. The frequency of establishment was 9.6%.Five of these ES cell lines were demonstrated karyotypically normal with diploidcomposition of over 70%. They shared the characteristics of early embryonic cells:positive for alkaline phosphatase activity and oct4 gene expression. They also showedhigh potency to differentiate into wide types of cells in vivo. Following injection ofthe blastocysts, three of them showed abilities to give rise to chimeras and MESPU35cell line was identified to have high efficiency of colonization into the germline. Theclones of MESPU35 cells maintained the germline competence and the mutant clonealso retained the high potency to participate into the development of embryos.MESPU35 cells can serve as a valuable vehicle for the produchon of mutant mice.更多还原