【摘要】 目的　研究苜蓿皂甙对实验大鼠胆固醇排泄和人脐静脉内皮细胞释放一氧化氮 (NO)的影响。方法实验动物分为正常对照组、高胆固醇血症组、氯贝特 (又名安妥明 )阳性对照组和苜蓿皂甙组。测定各组肝脏胆固醇、胆汁总胆酸和胆汁胆固醇含量的变化 ;测定粪便中固醇净排出量的变化并比较分析。内皮细胞分为正常对照组和苜蓿皂甙处理 1h、2h、4h组。测定各组内皮细胞释放NO含量的变化并比较分析。结果　与高胆固醇血症组比较 ,苜蓿皂甙组肝脏胆固醇显著降低 ,胆汁总胆酸含量显著增加 ;苜蓿皂甙组粪便中总固醇和酸性固醇净排出量均显著增加。与正常对照组比较 ,苜蓿皂甙处理 1h、2h、4h各组NO的释放均显著增加。结论　苜蓿皂甙能够明显促进实验大鼠胆固醇的排泄 ;促使正常培养内皮细胞NO的释放。提示苜蓿皂甙可能是一种远期疗效较好、并能促使动脉粥样硬化斑块消退的降脂药物更多还原

【Abstract】 Objective To study the effects of alfalfa saponin on rat cholesterol excretion and nitric oxide(NO) release from human umbilical vein endothelial cells (HUVEC).Methods The liver and bile cholesterol,total bile acid and the cholesterol excretion in the experimental hypercholesterolemic rats were determined.The level of NO was observed in HUVEC.Results Compared with hypercholesterolemia group,the liver cholesterol was significantly reduced( P <0.01),the total bile acid in bile was significantly increased( P <0.01) and the total steroids and acidic steroids in feces were significantly increased in alfalfa saponin group.Compared with normal control, the NO release of HUVEC was significantly increased in Alfalfa saponin group after treatment for 1 h,2 h or 4 h. Conclusion Alfalfa saponin can increase the cholesterol excretion in experimental rats and the NO release in HUVEC.Alfalfa saponin might be a promising hypocholesterolemic drug.更多还原