【摘要】 为探讨急性放射损伤小鼠骨髓细胞粘附分子表达及川芎嗪对其影响，将27只昆明小鼠平均分为3组：正常组，急性放射损伤对照组，川芎嗪组。将对照组及川芎嗪组小鼠用8．0Gy60Coγ射线照射后，分别胃饲生理盐水及25mg／ml川芎嗪注射液0．2ml／只，每d2次，连续6d。第7d用流式细胞仪检测所有小鼠骨髓单个核细胞CD49d、CD49e的表达水平，用免疫组织化学SABC方法检测骨髓基质细胞VCAM－1的表达、结果发现急性放射损伤对照组CD49d、VCAM－1的表达显著低于正常组（P＜0．01，P＜0．001），川芎嗪组CD49d、CD49e、VCAM－1的表达均显著高于对照组（P＜0．01，P＜0．001），且CD49d、CD49e高于正常组。结果显示川芎嗪能增强急性放射损伤小鼠骨髓造血细胞和基质细胞粘附分子的表达，有利于造血细胞的增生。更多还原

【Abstract】 To explore the effect of Ligustrazine on the expression of adherent molecule of bone marrow cells in acute radiation injured mice, 27 Kunmin mice were randomly divided into 3 groups: Normal group, acute radiation injured control group, Ligustrazine group. The mice in control and Ligustrazine groups were generally irradiated by 8. 0 Gy 60Co γ-ray, each mouse was respectively given through stomach 0. 2 ml normal saline or 5 mg Ligustrazine twice a day for 6 days. On the 7th day, expressions of CD49d and CD49e in bone marrow monomuclear cells (MNC) were detected by flow cytometry, and VCAM-1 on stromal cells by immunohistochemisty SABC method. The results showed that expression of CD49d and VCAM-1 in radiation injured group were significantly lower than in normal group (P<0. 01, P<0.001 respectively). Expression of CD49d, CD49e and VCAM-1 were much higher in ligustrazine treated group than in the control group (P<0. 01, P<0. 001respectively), and CD49d and CD49e higher than in the normal group. It was concluded that Ligustrazine could increase the expression of adherent molecule of bone marrow cells and stromal cells in acute radiation injured mice, which was beneficial to the growth of hematopoietic cells.更多还原