【摘要】 为探讨萘酚喹、甲硝唑、诺氟沙星单用及其与青蒿琥酯伍用的体外抗疟作用，比较青蒿琥酯对恶性疟原虫敏感株与抗性株的差别。应用Ｒｉｅｃｋｍａｎｎ体外微量法测得萘酚喹、甲硝唑、诺氟沙星、青蒿琥酯对敏感株的ＩＤ５０分别为８８．５、１３０３．３、１２１５．９及９．６ｎｍｏｌ／Ｌ；对抗性株的ＩＤ５０依次为１１９．４、３５８７９．６、９８１８．２及８５．１ｎｍｏｌ／Ｌ。在青蒿琥酯与这３种药物伍用中，青蒿琥酯的ＩＤ５０分别为２０．０、３３．０及５．０ｎｍｏｌ／Ｌ，单用组较之分别高出３．３、１．６及１６．０倍；其他３种药物的ＩＤ５０依次为１５．７、１９８．６、３４．０ｎｍｏｌ／Ｌ，单用组较之高出６．６、１９７．７及２８７．８倍。结果提示抗青蒿琥酯恶性疟原虫对甲硝唑、诺氟沙星有交叉抗性的征兆；青蒿琥酯分别与诺氟沙星、甲硝唑、萘酚喹伍用对抗青蒿琥酯恶性疟原虫有明显增效作用。更多还原

【Abstract】 Rieckmann in vitro microtechnic assay were applied to explore the effect of naphthoquine, metronidazole and norfloxacine and their combination with artesunate to artesunate sensitive or resistant Pfalciparum strains. The ID50 of sensitive strain to naphthoquine, metronidazole, norfloxacine and artesunate were 88.5, 1303.3, 1215.9 and 9.6 nmol/L and of resistant strain 119.4, 35879.6, 9818.2 and 85.1 nmol/L respectively. The ID50 of artesunate combination with naphthoquine, metronidazole and norfloxacine were 20.0, 33.0, and 5.0 nmol/L. The ID50 of single drug groups were 3.3, 1.6, 16.0 times higher than that of artesunate combination groups. The ID50 of the other 3 drugs were 15.7, 198.6, and 34.0 nmol/L. Single drug groups were 6.6, 179.7 and 287.8 times higher than that of the 3 drugs. The results showed that artesunate resistant P.falciparum revealed crossresistance to metronidazole and norfloxacine and that artesunate combination with naphthoquine, metronidazole and norfloxacine had obviou更多还原