【摘要】 目的研究戈米辛Ｊ对铜离子和内皮细胞诱导的低密度脂蛋白氧化修饰的作用。方法以巴比妥酸反应物质、荧光物质及电泳迁移率测定低密度脂蛋白的氧化程度。结果戈米辛Ｊ抑制铜离子诱导的ＬＤＬ氧化修饰，有效质量浓度为５μｍｏｌ／Ｌ，ＩＣ５０（９５％可信限）为２６．２（１６．５～４１．５）μｍｏｌ／Ｌ，尚可抑制氧化的低密度脂蛋白产生的荧光物质及琼脂糖凝胶电泳迁移率；对内皮细胞诱导的低密度脂蛋白氧化修饰，戈米辛Ｊ的抑制作用呈剂量依赖关系。结论戈米辛Ｊ对铜离子和内皮细胞诱导的低密度脂蛋白氧化修饰有显著抑制作用。更多还原

【Abstract】 Purpose To study the effect of gomisin J on the oxidative modification of low density lipoprotein(LDL) induced by copper and endothelial cells(EC). Methods Oxidative modification of LDL was examined by thiobarbituric acid reactive substances(TBARS),lipofusion and electrophoretic mobility. Results Gomisin J inhibited copper induced LDL peroxidation with IC 50 (95% confidence limits)26.2(16.541.5) μmol/L。It could also inhibit the products of lipofusion and the rate of eletrophoretic mobility of oxidized LDL (ox LDL).Additionally,gomisin J showed an inhibitory effect on EC induced ox LDL in a dose dependent manner. Conclusions Gomisin J inhibits the oxidative modification of LDL induced by copper and EC.更多还原