【摘要】 以鸡传染性法氏囊病病毒（ＩＢＤＶ）强毒株为对象研究了高压力对ＩＢＤＶ活性的影响。以鸡胚成纤维细胞和ＳＰＦ雏鸡为模型的研究表明，ＩＢＤＶ的感染活性随压力和作用时间的增加而降低，直至完全丧失。但高压力失活的ＩＢＤＶ可有效诱导雏鸡血清抗体大量产生，对雏鸡的保护作用达１００％，且无发病现象。说明ＩＢＤＶ经一定高压力条件处理后仍具有高免疫原性，即具备了疫苗的特性。内源荧光光谱、内源荧光偏振、散射光及ｂｉｓＡＮＳ结合性质测定均表明高压力作用过程中ＩＢＤＶ结构发生了变化，与寡聚蛋白的高压下解离聚合过程有明显相似性。电镜观察亦显示高压力处理前后ＩＢＤＶ形态发生了明显变化。这些结果为解释高压力作用后ＩＢＤＶ具备疫苗特性的机制提供了重要信息。更多还原

【Abstract】 Effects of high hydrostatic pressure on the activity of infectious bursal disease virus(IBDV) was described. The infectivity of IBDV decreased with the increasing of pressure and pressurizing time and may be completely lost, indicated by the tests with chicken embryo fibrablast cell and young chicken as models. How ever, pressure inactivated IBDV still remained high immunogenicity and might induce high levels of protecting serum antibody. Fluorescence spectroscopy showed obvious differences between the structures of native and pressurized IBDV, similar with the dissociation reassociation of oligomeric proteins under high pressure. The changes of pressurized IBDV were also directly observed with electron microscopy. These results suggest that pressure inactivated IBDV may be used as a vaccine.更多还原