【摘要】 利用脂质体模型膜系统研究了跨膜电位对蜂毒肽在磷脂膜上取向的影响。采用高效液相色谱与质谱技术相结合方法， 分析了蜂毒肽与磷脂膜作用后的胰蛋白酶酶解产物。结果发现， 当蜂毒肽分子与没有跨膜电位的脂质体结合后， 它的所有酶切位点都能够被酶有效切断； 当蜂毒肽与带负向膜电位的脂质体结合后， 其Ｎ 端的一个酶切位点被封闭。从而说明跨膜电位造成了蜂毒肽在膜上的重新取向更多还原

【Abstract】 The orientation of melittin binding to liposomes with or without transmembrane potential was investigated. With a combination of high performance liquid chromatography and mass spectrometry, the membrane association state of melittin was detected by analyzing its trypsin digested products. It was found that the enzyme could access all the proteolytic sites on protein binding to the liposome without membrane potential, while one proteolytic site on the N terminal of the protein was blocked after it binding to the liposome with negative transmembrane potential. The results showed that melittin changed its orientation in the latter case.更多还原