【摘要】 γδＴ淋巴细胞在人外周血单个核细胞中的含量不超过１０ ％ ， 而研究γδＴ淋巴细胞的功能需要大量的γδＴ淋巴细胞。为此， 采用磁珠（ｍａｇｎｅｔｉｃ ｍｉｃｒｏｂｅａｄｓ）阳性选择（ｐｏｓｉｔｉｖｅｓｅｌｅｃｔｉｏｎ） 法分离和纯化γδＴ淋巴细胞， 纯化可达９９％ 左右； 同时对富集的γδＴ淋巴细胞在体外的增殖和细胞毒效应进行观察， 此法快速、有效、纯度高， 对γδＴ淋巴细胞功能影响较小。为γδＴ淋巴细胞的研究和应用提供了便利。更多还原

【Abstract】 Although γδT lymphocytes make up no more than 10% of the peripheral blood mononuclear cells,they appear to play an important role in tumour cytotoxicity In order to study the function of γδT lymphocytes and their response to nonpeptide antigens,a new method for isolation of γδT lymphocytes from peripheral mononuclear blood cells was established γδT lymphocytes were coated by magnetic microbeads conjugated to goat anti mouse IgG in combination with murine anti human TCR γδ McAb,and were then isolated with the use of a magnetic cell sorter The maximal purity of the γδT lymphocytes was up to 99 48% The natural killer activity of the γδT lymphocytes as well as proliferative response induced by interleukin 2 and MEP were not affected by isolation procedure or the presence of the magnetic microbeads on the γδT lymphocytes Therefore,the described method might be a useful tool for further characterization of γδT lymphocytes and can easily be applied for clinical use in adoptive immunotherapy trials with isolated γδT lymphocytes更多还原