【摘要】 以建兰花叶病毒海南分离物RNA为模板，通过RT－PCR扩增其外壳蛋白基因，克隆并测序。测出此基因大小为666nt，核苷酸顺序与文献报道序列同源性为88．8％～96．1％，推算出的氨基酸同源性为75．6％～91．0％，外壳蛋白亚基氨基酸顺序差异主要在近C-端部分。另外，利用反转录-PCR法检测香草兰上的建兰花叶病毒。更多还原

【Abstract】 The coat protein gene of cymbidium mosaic virus Hainan isolate was amplified from the extracted viral RNA with reverse transcription-polymerase chain reaction method. The result proved that the gene contains 666 nt. Compared with four CyMV isolates reported, it shares 88.8～96.1% and 75.6～91 .0 % identity at the sequence levels of nucleotide and deduced amino acid respectively. The main difference of amino acid sequences of their coat proteins is in the region near C-terminal of protein. CyMV infected vinilla plants could be detected with RT-PCR method.更多还原