【摘要】 目的:了解南京地区丙型肝炎病毒(HCV) 感染及乙型肝炎病毒(HBV) 抗原亚型分布情况。方法:对1993 ～1995 年住院肝病患者2 157 例,应用RT- PCR、HCV 进行基因型、阳性克隆序列分析及HBV亚型的检测。结果:HCVRNA 阳性率为8.7 % 。对100 例HCV 基因型分析:Ⅱ型占91% ,Ⅲ型占6% 。HBV亚型分析:adr 占55.6% ,adw 占38.4% 。2 例HCV 阳性克隆均为Ⅲ型,与日本株(HC- J5 、HC-J6)密切相关。结论:中国南京的HCV可能是近代从日本传入的,HBV亚型以adr、adw 为主更多还原

【Abstract】 Objective:The purpose of the investigation was to study the molecular epidemiology of hepatitis C virus(HCV) genotype and subtype of HBsAg in Nanjing.Methods:The sera from 2?157 liver disease patients were analyzed.HCV RNA was detected by polymerase chain reaction(PCR).The samples positive for HCV RNA were subject to the genotype analysis.The PCR product was then cloned into the plasmid vector.Their nucleotide sequence was determined by dideoxynucleotide chain termination method.Phylogenetic trees were calculated by the neighbor-joining method.Results:HCV RNA was positive in 188(8.7%) of 2?157 patients.HCV genotypes were observed in 100 of the HCV?RNA positive sera,genotypes Ⅱ and Ⅲ were 91% and 6%,respectively.The HCV isolate from two Nanjing-born patients was closely related to that in Japan.The adr and adw were found to be 55.6% and 38.4%,respectively in serum samples by HBsAg subtype identification.Conclusions:It is supposed that HCV infection in Nanjing was brought there recently from Japan.The adr and adw are main subtypes in HBsAg.更多还原