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大赖草第2,7,14条染色体在小麦背景中的传递行为

Transfer behavior of Leymus racemosus Lr2,Lr7,Lr14 chromosome in common wheat background

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【作者】 陈发棣; 陈佩度; 刘文轩;

【Author】 Chen Fadi,Chen Peidu and Liu Wenxuan (Key Lab of Crop Cytogenetics,Ministry of Agriculture,Nanjing Agric Univ,Nanjing 210095)

【机构】 南京农业大学农业部作物细胞遗传重点开放实验室!南京210095;

【摘要】 配制普通小麦-大赖草Lr2、Lr7、Lr14 染色体二体异附加系(92G169、93G52-8、94G15) 与普通小麦-簇毛麦6VS/6AL易位系 (92R149) 3 个杂交组合, F1 分别与扬麦5 号正反杂交和自交。对正反杂交后代和F2 代个体进行有丝分裂中期染色体C-分带, 分析大赖草Lr2、Lr7和Lr14 染色体在F1 代的传递行为。结果为: Lr2随雄配子和雌配子的传递频率分别为27.3% 和17.9% , 在92G169×92R149F2 群体中,Lr2 染色体单体添加个体占29.9% ,二体添加个体占4.3% ; Lr7随雄配子和雌配子的传递频率分别为28.6% 和15.6% , 在 93G52-8×92R149F2 群体中, Lr7 染色体单体添加个体占30.1% , 二体添加个体占4.3% ; Lr14 随雄配子和雌配子的传递频率分别为71.4% 和29.5% , 在94G15×92R149F2 群体中, Lr14 染色体单体添加个体占59.8% , 二体添加个体占20.5% 。表明大赖草Lr2、Lr7、Lr14染色体在小麦背景中的传递行为存在较大差异, 三条染色体随雄配子的传递频率均高于随雌配子的传递频率, 其中Lr14 染色体随雄配子有优先传递的可能。

【Abstract】 Hybridizations were made between Triticum aestivum L.racemosus Lr2,Lr7,Lr14 disomic addition lines(92G169,93G52 8,94G15)and T.aestivum Haynaldia villosa 6VS/6AL translocation line(92R149)and F 1 hybrids were selfed and reciprocally crossed with Yangmai 5.Chromosome C bandings of RTCs were conducted on all these hybrids to study the transfer behavior of L.racemosus Lr2,Lr7 amd Lr14.The results showed that the transfer frequency along androgamete of Lr2,Lr7 and Lr14 chromosome was 27 3%,28 6% and 71 4%,along gynogamete was 17 9%,15 6% and 29 5% respectively.Meanwhile,Lr2 monosomic addition and disomic addition individuals were 29 9% and 4 3% respectively in F 2 generation between 92G169 and 92R149.In 93G52 8×92R149 F 2 group,Lr7 monosomic addition and disomic addition plants separetely took 30 1% and 4 3%.But the results of the same type about Lr14 were 59 8% and 20 5% in F 2 group of 94G15×92R149.Above information indicated that the transfer behavior of L.racemosus Lr2,Lr7 and Lr14 chromosome was different.Androgamete transfer frequency was higher than that of gynogamete about all these three chromosomes,and Lr14 chromosome was probably preferential in transfer along androgamete. ABSTRACT Hybridizations were made between Triticum aestivum L.racemosus Lr2,Lr7,Lr14 disomic addition lines(92G169,93G52 8,94G15)and T.aestivum Haynaldia villosa 6VS/6AL translocation line(92R149)and F 1 hybrids were selfed and reciprocally crossed with Yangmai 5.Chromosome C bandings of RTCs were conducted on all these hybrids to study the transfer behavior of L.racemosus Lr2,Lr7 amd Lr14.The results showed that the transfer frequency along androgamete of Lr2,Lr7 and Lr14 chromosome was 27 3%,28 6% and 71 4%,along gynogamete was 17 9%,15 6% and 29 5% respectively.Meanwhile,Lr2 monosomic addition and disomic addition individuals were 29 9% and 4 3% respectively in F 2 generation between 92G169 and 92R149.In 93G52 8×92R149 F 2 group,Lr7 monosomic addition and disomic addition plants separetely took 30 1% and 4 3%.But the results of the same type about Lr14 were 59 8% and 20 5% in F 2 group of 94G15×92R149.Above information indicated that the transfer behavior of L.racemosus Lr2,Lr7 and Lr14 chromosome was different.Androgamete transfer frequency was higher than that of gynogamete about all these three chromosomes,and Lr14 chromosome was probably preferential in transfer along androgamete. Hybridizations were made between Triticum aestivum L.racemosus Lr2,Lr7,Lr14 disomic addition lines(92G169,93G52 8,94G15)and T.aestivum Haynaldia villosa 6VS/6AL translocation line(92R149)and F 1 hybrids were selfed and reciprocally crossed with Yangmai 5.Chromosome C bandings of RTCs were conducted on all these hybrids to study the transfer behavior of L.racemosus Lr2,Lr7 amd Lr14.The results showed that the transfer frequency along androgamete of Lr2,Lr7 and Lr14 chromosome was 27 3%,28 6% and 71 4%,along gynogamete was 17 9%,15 6% and 29 5% respectively.Meanwhile,Lr2 monosomic addition and disomic addition individuals were 29 9% and 4 3% respectively in F 2 generation between 92G169 and 92R149.In 93G52 8×92R149 F 2 group,Lr7 monosomic addition and disomic addition plants separetely took 30 1% and 4 3%.But the results of the same type about Lr14 were 59 8% and 20 5% in F 2 group of 94G15×92R149.Above information indicated that the transfer behavior of L.racemosus Lr2,Lr7 and Lr14 chromosome was different.Androgamete transfer frequency was higher than that of gynogamete about all these three chromosomes,and Lr14 chromosome was probably preferential in transfer along androgamete.

【关键词】 大赖草; 染色体; 传递行为; C-分带;
【Key words】 Leymus racemosus; chromosome; transfer behavior; C banding;
  • 【文献出处】 南京农业大学学报 ,JOURNAL OF NANJING AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1999年04期
  • 【分类号】S512.035.1
  • 【被引频次】7
  • 【下载频次】97
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