【摘要】 本文采用了能反映流体轴向扩散的大型液相色谱分离过程数学模型，应用计算机模拟分析蔗糖和还原糖的色谱分离过程，从吸附剂吸附容量和柱装填密度两个方面；考察柱吸附容量对大型色谱分离的产率和回收率的影响。研究结果表明：在色谱柱中流体线速度恒定的条件下对多组分分离，回收率是随吸附剂吸附容量以及床层装填密度的增大而增大的；产率先随吸附剂吸附容量的增大而增大，而在出现峰值后下降；随着床层装填密度的增大，产率增加，但与此同时轴向扩散系数也增大，从而降低分离效率，导致在较高装填密度的范围内产率增力。的幅度减少。在相同的吸附剂用量下，采用短柱高装填密度的色谱柱将比长柱低装填密度色谱柱能获得更高的回收率和产率。更多还原

【Abstract】 In the paper, the effects of adsorption capacity of adsorbents and packing density which are two aspects of adsorption capacity of column on the production rate and recovery of chromatographic separation are investigated by applying large scale chromatography model and simulating the process of the chromatographic separation of sucrose and reducing sugar. Results show that under the condition of having fluid velocity constant, recovery increases with the increase of the adsorption capacity of the adsorbents and the increase of the packing density of the column, with the increase of the adsorption capacity of the adsorbents, production rate increases gradually but it will decrease after reaching the peak of the production rate, and with the increase of the packing density of the column, the production rate increases, and on the other hand the axial dispersion coefficient increases, which decreases separation efficiency and therefore results in the increase in the production rate to become small within the extent of high packing density of the column. Under the condition of the same amount of the adsorbents used,the comparisons between the short column with high packing density and the long column with low packing density when they are used in chromatographic separation are made. Result indicates that the use of the former can get higher production rate and higher recovery than the use of the latter.更多还原