【摘要】 分析了69个随机挑选的核盘菌弱毒菌株Ep－1PN原生质体再生后代的生长速度、菌落形态和毒性。结果表明：其中的35个再生后代在PDA平板上均匀扩展，形成典型的核盘菌菌落，生长速度大于18mm／d。在离体莴苣杆上，这些后代的培养物引起较大的病斑。当它们同亲合性菌株Ep-1PNA183在PDA平板上接触时没有发现Ep-1PNA183菌落发生异常变化，说明这些后代已失去了Ep－1PN的弱毒因子，恢复了正常核盘菌的特性。10个再生后代同Ep－1PN的生长及致病性等没有差异。其他的24个再生后代在菌丝生长速度、菌落形态和毒性等方面介于Ep-1PN和35个完全恢复的后代之间，因而是部分恢复的后代。这些后代的获得为进一步研究核盘菌弱毒现象的分子机理奠定了基础。更多还原

【Abstract】 Sixty-nine protoplast-regenerated cultures of Sclerotinia sclerotiorum hypovirulent isolate Ep-1PN were ran-domly obtained from the regeneration medium plates. Their growth rates, colony morphologies and virulence were examined.The results showed that: (1)Thirty-five protoplast-regenerated cultures were thought to be totally recovered because they grew radically on PDA plate and formed typical colony morphologies with normal growth rates (more than 18 mm/d), they could in-duce large lesions on detached lettuce top stems, but could oot cause any detectable effects on the growth of strain Ep-1PNA183, an isogenic and virulent strain of Ep-1PN; (2)Twenty-four protoplast-regenerated cultures were thought to recover to different degrees because they differed in growth rates, colony morphologies and virulence for strain Ep-1PN. They could cause the hyphal tips of strain Ep-1PNA183 to branch excessively when paired with them; (3)Other ten prootoplast-regenerated cultures were not significantly different from strain Ep-1PN.更多还原