【摘要】 测定急性脊髓损伤（ＳＣＩ）后大鼠血浆和脊髓组织中内皮素（ＥＴ）及电解质含量，了解其变化规律， 探讨其作用机制。方法：用放免法测定不同程度脊髓损伤后１ｈ，４ｈ，８ｈ，２４ｈ血浆和脊髓组织内皮素含量，并测定 相应脊髓组织钠、钾、钙离子含量和含水量。结果：损伤１ｈ，４ｈ，８ｈ血浆内皮素含量比对照组高（Ｐ＜０．００５），２４ｈ与 对照组比较差异无显著性（Ｐ＞０．０５）。脊髓组织内皮素含量实验组均较对照组高（Ｐ＜０．０５），同一时间不同程度损 伤组比较，重度损伤组均较中度损伤组高（Ｐ＜０．０５）。脊髓组织中钠、钾、钙离子含量及含水量与组织内皮素含量 变化趋势一致。结论：急性脊髓损伤后脊髓组织内皮素含量升高，且与损伤程度有关，组织内皮素含量升高可导致组织水肿，钙、钠离子内流及钾离子外流而加重损伤。更多还原

【Abstract】 Aim:To investigate the rule of changes of the contents of endothelin(ET)and electroyte in plasma and spinal cord tissues after spinal cord injury(SCI).Methods:The contents of ET in the plasma and the spinal cord were measured by radioimmunoassay (RIA)1,4,8,and 24 hour after SCI,and the contents of water,Ca2+,Na+and K+ in the spinal cord were measured at about-mentioned time periods.Results: The ET contents of the plasma in experimental groups were higher than those in the control group at 1,4 and 8hour after injury(P<0.05),but not at 24 hours later(P>0.05).The ET contents of the spinal cord in all the experimental groups were higher than those in the control group(P<0.05).The ET contents in the severely injured groups were higher than those in the moderately injured groups(P<0.05).The trend of changes of the contents of Ca2+,Na+, K=and water was in accordance with that of ET in the spinal cord.Conclusion:The increase of the ET contents of spinal cord is related to SCI degree,suggesting that ET can aggravate the injury by inducing an intracellular shift of Ca2+ and Na|,extracellular shift of K+and edema of tissues.更多还原