【摘要】 目的:研究佛波酯在药物预处理中是否对离体兔心具有保护。方法:采用Langendroff离体兔心灌注模型。C组为对照组即实验模型。IPC组:在长缺血前,兔心停灌5 min,复灌10 min。PPC组:在长缺血前,兔心灌注0.01 nmol/LPMA 5 min,冲洗10 min。分别在缺血前和灌注末测LVESP和LVEDP,计算恢复率代表心功能。在灌注末测心肌中CK-MB手MDA。资料用q检验。结果:①心功能:IPC组、PPC组均高于C组(P<0.05),PPC组高于IPC组(P<0.05)。②生化:IPC组、PPC组优于C组(P<0.05),PPC组优于IPC组(P<0.05)。结论:佛液酯对兔心具有较好的保护作用。更多还原

【Abstract】 Aim: The present sudy investigated whether phorbol myrisate acetate(PMA) plays a role in pharmacologic preconditioning in isolated rabbit heart. Method: In the present study, Langendroff isolated rabbit heart model was used. Group C was control group (Langendroff model). Group IPC: The rabbits were subjected to ischemia 5 minutes and reperfusion 10 minutes before long global ischemia. Group PPG: PMA(0.01 nmol/L) was infused 5 minutes and then washed out 10 ninutes before the onset of long global ischemia. Before long ischemia and at the end of reperfusion, LVESP,LVEDP and the contents of CK- MB,MDA in cardiac tissues were measured, respectively. AU values were tested with q test. Results:(1) Cardiac function: The parameters in Group IPC and Group ppc were better than those in Group c( P < 0.05) . Group PPC was better than Group IPC (P< 0.05) .(2)Biochemical parameters of myocardial tissues:Group IPC,Group PPC were better than Group C(P<0.05). Group PPC was better than Group IPC( P < 0.05). Conclusion: PMA has myovardial protective effect.更多还原