【摘要】 研究了几种蛋白酶对桑蚕蛹蛋白粉的水解情况 ,采用均匀设计法对桑蚕蛹蛋白粉的限制水解条件进行了优化选择 ,得到胰酶水解桑蚕蛹蛋白粉的优化条件为 :酶用量占底物的质量分数为 4 50 % ,底物含量的质量体积分数为 1 5% ,反应温度为 4 0℃ ,水解时间为 6 0h ,水解pH值为 8 0 ,在此条件下水解 ,酸溶性肽得率可达 92 3 7% .更多还原

【Abstract】 An optimum technology of peptide preparation from silkworm pupa protein by means of hydrolysis with pancreatin was studied through uniform design.The optimum conditions for the limited hydrolysis of silkworm pupa protein were concluded as follows:the ratio of enzyme to substrate ( E/S )is 4.5%( W/W ),the substrate concentration ( S ) is 1.5% ( W/V ),the recreation occurs at 40℃ and pH 8.0 for 6 h.And the yield of peptide is 92 37 %.更多还原