【摘要】 正交设计试验结果表明，青霉菌（Ｐｅｎｉｃｉｌｌｉｕｍ ｓｐ．ｍ８） 胞外木聚糖酶活力达９２．９６ Ｕ／ｍＬ，合适的产酶发酵条件为：培养基（ｇ／Ｌ），麦草粉４０，（ＮＨ４）２ＳＯ４ ４．４４，ＫＨ２ＰＯ４ １．０，ＭｇＳＯ４ ·７Ｈ２Ｏ０．５，ＮａＣｌ０．３，Ｔｗｅｅｎ ８０ ３．０，ＣａＣＯ３ １．０；每亳升培养基接种２．０×１０５ 个孢子，２８ ℃，水浴振荡培养４ ｄ（１２０ｒ／ｍｉｎ）。更多还原

【Abstract】 An orthogonal experiment was carried out for optimization of fermentation of Penicillium sp. m8 in order to produce extracellular xylanases. The xylanase activity of culture filtrate of m8 was 92.96 U/mL. The medium(g/L) contained wheat straw powder 40,(NH4)2SO4 4.44, KH2PO4 1.0,MgSO4·7H2O 0.5,NaCl 0.3,Tween 80 3.0, CaCO3 1.0. 1.0×107 spores were inoculated into 50 mL medium in 250 mL Erlenmeyer Flask in a water rocker(120 r/min,28 ℃)which stirred for 4 days.更多还原