【摘要】 通过碱变性法提取线粒体DNA(mtDNA),用地高辛标记的探针Southern杂交进行限制性酶切多态性(RFLP)分析,研究中国褐家鼠Rattusnorvegicus遗传多态性。采用ApaⅠ、AvaⅠ、BamHⅠ、BclⅠ、BglⅠ、ClaⅠ、EcoRⅠ、EcoRⅤ、HindⅢ、PvuⅡ、ScaⅠ和XbaⅠ等12种限制性内切酶分析来自我国8个地区26只褐家鼠的线粒体DNA,共检出20种限制性态型(morph)和11种mtDNA单倍型。其中BamHⅠ、EcoRⅤ、ScaⅠ和BclⅠ等5种限制酶检测到多态性,限制性态型间差异1～2个切点。各单倍型间的遗传距离0002～0014,平均遗传距离00095。根据已发表的资料,计算出我国褐家鼠与实验大鼠之间的遗传距离约00087。结果表明我国褐家鼠在mtDNA水平呈现广泛的种内遗传多态性,个体间、群体内和群体间存在一定的遗传变异,群体内和群体间的遗传分化程度处于相近的水平。更多还原

【Abstract】 Twenty six samples of Rattus norvegicus from 8 localities in China were used for the investigation of the genetic polymorphism by means of mitochondrial DNA (mtDNA) RFLP analysis.The mtDNA of each sample was digested with 12 restriction endonucleases, Apa Ⅰ, Ava Ⅰ, Bam HⅠ, Bcl Ⅰ, Bgl Ⅰ, Cla Ⅰ, Eco RⅠ, Eco RⅤ, Hin dⅢ, Pvu Ⅱ, Sca Ⅰ and Xba Ⅰ.The fragment patterns were examined by agarose gel electrophoresis or by Southern hybridization with DIG labeled mtDNA probe.Twenty restriction morphs have been recognized,which can be grouped into 11 haplotypes.Extensive mtDNA polymorphism was found between individual animals,within and among populations of Chinese R norvegi cus .Almost each of populations had distinct mtDNA haplotype,some with more than one type.The divergence for the 11 detected haplotypes ranged from 0 2% to 1 4%,comparable to that previously reported for the rats from the United States,Japanese and the laboratory type.The findings are helpful for understanding the dispersion and evolution of this animal.更多还原