【摘要】 采用流式细胞仪测定Ｔ细胞亚群和脾脏Ｂ淋巴细胞电泳对应用活化剂ＨＹＰ协同“４２５”制剂的日本血吸虫感染的ＢＡＬＢ／ｃ小鼠进行细胞和体液免疫机能测定，并与单一用“４２５”制剂和感染对照动物进行比较。实验结果表明，ＨＹＰ协同“４２５”制剂应用后２ｗｋ，相当于感染后６ｗｋ，ＣＤ４＋／ＣＤ８＋淋巴细胞亚群的比值为６．８２，显著高于后两组的１．８７和１．７９，脾脏中Ｂ淋巴细胞总频率达７５％，高于单一用“４２５”制剂和感染对照组的６２．５％和６４．５％，特异性抗体水平（ＯＤ均值）为０．５５±０．１１，分别高于后两组的０．３５±０．０３和０．３７±０．０７。应用后３～４ｗｋ，相当于感染后７～８ｗｋ，ＣＤ４＋／ＣＤ８＋比值为１．９８～３．４７，后两组则分别为１．３２～１．５９和１．４８～１．７０，Ｂ淋巴细胞数３组均相近，但前组抗体水平（ＯＤ均值）的升高率（８２．２２％～９１．１８％）仍高于后两组。由此提示，活化剂ＨＹＰ协同“４２５”制剂诱导出的抗日本血吸虫虫卵肉芽肿的免疫效应，显著减弱血吸虫虫卵肉芽肿病变是与增强其细胞和体液免疫功能密切相关，具有明显的理论意义，且为进一步探索抗肉芽肿病变提供了重要依据更多还原

【Abstract】 The specific T lymphocyte subpopulations, B lymphocyte and specific antibody were detected at 2nd to 4th weeks after BALB/c mice were injected with activator HYP coordinating "425" reagent (group Ⅰ), "425" reagent only (group Ⅱ) and the infected as control (group Ⅲ) . The percentages of CD4 + in mice in group Ⅰ( 42.68 %±6.54%￣25.36%±1.31%) were much higher than those in group Ⅱ (22.34%±2.56%￣20.00%± 1.44 %) and group Ⅲ (23.40%±3.90%￣21.62%±2.50%) during this period. The ratios of CD4 +/CD8 +(6.86￣3.47) were much higher than those in group Ⅱ(1.92￣1. 59) and in group Ⅲ(1.72￣1.70). The percentages of B lymphocyte in mice in group Ⅰ(75%￣53.75%) were much higher than those in group Ⅱ(62.50%￣54.10%) and in group Ⅲ (64.50%￣50.16%). The specific antibody level( OD mean value) of sera from group Ⅰ(0.55± 0.11 ￣1.06±0.09) were much higher than those from group Ⅱ(0.35±0.03￣0.49±0.09) and group Ⅲ (0.37± 0.07 ￣0.55±0.77) mice. The findings suggested that activator HYP coordination "425" reagent can induce immune functions enough to diminish schistosomal granulomas.更多还原