【摘要】 目的研究脐动脉血流异常的胎盘绒毛、绒毛干血管和脐带一氧化氮合酶(nitric oxidesynthase,NOS)的分布及活性大小的变化,探讨胎儿胎盘循环中 NOS 在脐动脉血流波形异常发生中的作用。方法对9名脐动脉血流检测正常和8名脐动脉血流检测异常孕妇的胎盘绒毛、绒毛干血管和脐带组织,用还原型辅酶Ⅱ-黄递酶组织化学法(NADPH-d 组织化学法)研究 NOS 的组织分布,用比色法测定 NOS 活性。结果 NOS 主要分布于合体滋养细胞和血管内皮细胞中。正常组大多数合体滋养细胞中的蓝色颗粒聚集成团块,主要位于细胞的基底部;异常组大多数合体滋养细胞中的蓝色颗粒呈弥散状于细胞核周围,无基底部聚集趋势,着色强度明显较正常组弱。异常组胎盘绒毛组织中,NOS 活性显著降低。结论在脐动脉血流异常胎盘绒毛组织中 NOS 的活性降低且位置异常,脐动脉血流异常的发生可能与合体滋养细胞中 NOS 活性降低有一定关系。更多还原

【Abstract】 Objective To study the nitric oxide synthase(NOS)distribution and activity in placental villous,stem villous vessel and umbilical vessel from pregnancies with abnormal umbilical artery flow velocity waveforms and discuss the effect of NOS in the development of abnormal umbili- cal artery flow velocity waveforms.Methods Using NADPH-diaphorase histochemical method and colorimetry,we studied the distribution and activity of NOS in placental villous,stem villous vessel and umbilical vessel tissues from nine women with normal umbilical artery Doppler waveforms and eight women with abnormal umbilical artery Doppler waveforms.Results The NOS primarily dis- tributed in the syncytiotrophoblast and vessel endothelial cells.In the majority syncytiotrophoblast of normal group,the blue granules gathered to mass,appeared primarily basal in location.In the ma- jority syneytiotrophoblast of abnormal group,the blue granules diffused primarily around nucleus, the staining intension was weaker than normal.Placental villous from the abnormal group had signif- icantly lower activities of NOS than the normal group.Conclusions The lower activities and an abnormal distribution of NOS were found in the placental villous of women with abnormal umbilical artery flow velocity waveforms.The abnormity of NOS may contribute to the development abnormal umbilical artery flow velocity waveforms.更多还原