【摘要】 抗日本血吸虫生殖及卵胚发育免疫的研究是血吸虫疫苗研究的重要策略之一．本研究探讨了未成熟卵等不同抗原免疫诱导宿主产生抗日本血吸虫生殖及卵胚发育免疫的效果．研究证明SIEA26－28kDa抗原能诱导小鼠产生抑制雌虫生殖和卵胚发育成熟的免疫力．与对照组比较，SIEA26－28kDa抗原免疫鼠减虫虽不明显，但肝组织内每雌产卵数、成熟卵数和粪卵数（EPG）分别减少48．1％、83．6％、87．3％，死亡卵数明显增加（t=3．91，P＜0.01）．此外，未纯化的SIEA和纯化的SIEA26－28kDa、Sj31／32kDa抗原免疫组约见雌虫子宫内虫卵数下降，分别达40．9％、54．8％、53．2％，而成熟虫卵可溶性抗原（SEA）和SIEA－TGP抗原免疫未见上述故应．采用未成熟抗原或Sj31／32kDa、SIEA26－28kDa等三联抗原，分别对大动物（牲猪）进行免疫．与对照组比较，免疫猪肝脏表面虫卵结节的数量显著下降（86．5％），组织中虫卵数、粪卵数分别下降78.0％和90.6％．更多还原

【Abstract】 AIM:To observe the effect of anti-embryonation and anti-female fecundity immunity inmice immunized with soluble immature eggs antigen (SIEA) and the 26-28kDa antigens derived from SIEA of Schistosoma japonicum. METHODS: BALB/c mice were immunized withSIEA26-28kDa or SIEA-IGp antigen. The egg burdens in liver, intestines and faeces weretested on day 46 after challenge with cercariae of S. japonicum. And the size of the egg gramulomas in the liver after challenge was conpared between the immunized mice and unimmunized mice. RESULTS: The number of mature eggs in the livers, intestines and faeces wassignificantly reduced in mice immunized with SIEA26-28kDa by 83. 6%, 93. 3% and 87. 3%,respectively, and the number of dead eggs was much greater in the tissues of immunized micethan in that of control mice. The number and size of egg granuloma were significantly decreased in mice immunized with SIEA and SIEA26-28kDa, by 29. 26% and 49. 64%, respectively. The inhibitive effects on granuloma formation and spleen weights in the group immunized with SIEA26-28kDa were stronger than those in the group immunized with the crudeSIEA. In addition, the number of eggs in the uteri of female adults was obviously decreasedin mice immunized with SIEA26-28kDa antigens or with the SIEA. CONCLUSION:The 26-28kDa antigens were major components in SIEA and responsible for both anti-fecundity andanti-embryonation immunity.更多还原